Fig. 8. Fast aggregation of 
-catenin-negative HCT-8/R1 colon cancer cells is restored upon stable transfection with T-catenin cDNA. After preparation of single-cell suspensions, cell aggregation was measured by determination of the volume distribution (%) in function of the particle diameter (µm) at the starting point (t0) and after 30 minutes (t30). HCT-8/R1, HCT-8/E11R1 and HCT-8/E8 cells were all obtained by subcloning HCT-8 cells, but only HCT-8/E8 cells express endogenous E-catenin. HRpCN2 is a cloned transfectant of HCT-8/E11R1 cells expressing exogenous N-catenin (van Hengel et al., 1997); HCT-8/R1/T31 is a cloned transfectant of HCT-8/R1 cells expressing exogenous T-catenin. MB2 is a monoclonal E-cadherin blocking antibody.
