Fig. 1. Subcellular distribution of AKAP95 and RII in Reh, Reh-RII and Reh-RII(T54E) cells. (A) Assessment of AKAP95 and RII in Reh, Reh-RII and Reh-RII(T54E) cells. Lysates from interphase and mitotic cells (10⁶ cells per lane) were analyzed by immunoblotting using mAbs against human AKAP95 (mAb47) and human RII. (B,C) Immunofluorescence analysis of interphase (B) and mitotic (C) cells using affinity-purified anti-AKAP95 polyclonal antibodies (green) and anti-RII mAbs (red). DNA was counterstained with Hoechst 33342 (blue). Bars, 10 µm.

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