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Fig. 3. Association of AKAP95 and RII{alpha} at mitosis. (A) AKAP95 and RII{alpha} were immunoprecipitated (IP) from interphase (I) or metaphase (M) lysates of Reh-RII{alpha} and Reh-RII{alpha}(T54E) cells, and immunoprecipitates were immunoblotted using anti-AKAP95 and anti-RII{alpha} mAbs (Blot). (B) MNase-soluble chromatin (Chr) and a cytosolic fraction (Cyt) were prepared from mitotic Reh-RII{alpha} or Reh-RII{alpha}(T54E) cells. AKAP95 was immunoprecipitated and the precipitates immunoblotted using anti-RII{alpha} mAbs.





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