Fig. 4. Alkaline phosphatase disrupts the mitotic AKAP95-RII
interaction. (A) Anti-RII (first panel) and anti-AKAP95 immunoprecipitates (second panel) from interphase (I) and mitotic (M) Reh-RII and Reh-RII(T54E) cells were immunoblotted using anti-pT and anti-pS antibodies. (B) Soluble chromatin was prepared from mitotic Reh-RII cells and treated with 100 U ml-1 APase (APase+) or 100 U ml-1 APase plus 20 mM sodium vanadate (APase-). AKAP95 or RII were immunoprecipitated (IP) and immune precipitates immunoblotted using anti-AKAP95 and anti-RII mAbs. (C) Duplicates of blots were immunoblotted using anti-pT and anti-pS antibodies.
