Fig. 6. Integrin clustering and F-actin polymerization are both dependent on PKA. HT-1080 cells were treated with 20 µM MPKI peptide for 30 minutes and plated on poly-D-lysine coated cover slips for another 20 minutes in the presence of no antibody or 10 µg/ml 12G10 directly labeled with AlexaTM 568. F-actin was visualized with fluorescently labeled phalloidin (green) and ß1 integrins were visualized with AlexaTM 568-labeled 12G10 (red). Fluorescent images were combined to determine colocalization (yellow). Bar, 10 µm.
