Fig. 1. Cell-cycle-dependent change in localization of Citron-K in HeLa cells. HeLa cells in various phases of cell cycle were fixed. Endogenous Citron-K was stained with specific anti-Citron antibody (green) in the absence (left-hand pairs of panels) and presence (right-hand pairs of panels) of the antigenic peptide. Microtubules and DNA were stained with anti-ß-tubulin antibody (red) and TOPRO3 (blue), respectively. The left panels of each pair represent merged images. Specific signals can be identified by comparing the two pairs of panels. Nonspecific signals in the presence of the competing peptide appear the spectral overlap from strong tubulin staining. Note that endogenous Citron-K is detected as particulate staining in the cytoplasm in interphase cells (arrowheads), disperses in the cytoplasm in prometaphase, is transferred to the cortex in the cleavage furrow in telophase and is present in the midbody in post-mitotic cells. These intracellular signals disappear when the blocking peptide is present. Bars, 10 µm.
