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Fig. 2. Modulation of FAs and stress fiber structure after TGFß treatment. 3D shadow projections, views from the cell basal side. (A) Vinculin (red) and ß-cytoplasmic actin (green) organisation in control fibroblasts. FAs are small and some are connected with cytoplasmic actin stress fibers. Immature (arrow) and mature (arrowhead) FAs. (B) Vinculin (red) and {alpha}-SMA (green) organisation in fibroblasts treated for 5 days with TGFß. The size of vinculin-containing FAs is dramatically increased in parallel with the appearance of {alpha}-SMA in stress fibers. (C) Vinculin (red) and ß-cytoplasmic actin (green) organisation in fibroblasts after TGFß-treatment in the presence of rED-A. FAs are smaller and stress fibers are thinner than those in B. {alpha}-SMA is absent in stress fibers. Bar, 10 µm.





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