Fig. 3. Codistribution and 3D-structure (shadow projections) of vinculin and 
5ß1 integrin in FAs of control and TGFß-treated fibroblasts. (A) Immature (arrow) and mature (arrowhead) FAs of a control fibroblast (view from ventral side): 5ß1 integrin (red) is adjacent to vinculin (green) or colocalised with vinculin (yellow). (B,C) FAs after 5 days of TGFß treatment. (B) Vinculin staining is hidden by 5ß1 integrin staining (basal side of the cell). (C) Vinculin and 5ß1 integrin co-staining (yellow) in FAs appears after removing the first basal optical sections. The terminal portions of FAs appear surrounded by 5ß1 integrin. Bar, 10 µm.
