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Fig. 4. Organisation of ED-A FN and actin after TGFß treatment. 3D shadow projections. (A) ß-Cytoplasmic actin (red) and ED-A FN (green) in control fibroblasts. View from basal side of the cells showing that some terminal ends of actin stress fibers are connected with FN fibers. (B) {alpha}-SMA (red) and ED-A FN (green) in fibroblasts treated with TGFß. The appearance of {alpha}-SMA in actin stress fibers and their thickening are accompanied by an organisation of ED-A FN in parallel bundles and their association with the end of actin stress fibers. The first optical sections from basal surface of extracellular matrix adjacent to fibronexus have been removed. Bar, 10 µm.





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