Fig. 9. Effect of MAPK inhibitor SB230580 on ED-A FN polymerisation. Dupuytren’s (A,C) and lung fibroblasts (B,D) cultured in the presence of various concentration of FN and treated with 20 µM and 50 µM, respectively, of SB230580 for 4 days were either extracted by DOC to recover the polymerised FN fraction or solubilised in loading buffer. Polymerised ED-A FN was probed by western blot on DOC insoluble fraction using IST-9 antibody (A,B, upper line) and 
-SMA expression tested by western blot of whole cell extracts (A,B, lower line). The production of ED-A FN by the cells was tested by dot blot analysis of culture medium using IST-9 antibody. Western and dot blots were quantified by scanning densitometry to compare the level of soluble and polymerised ED-A FN (C,D).
