Fig. 1. Yeast two-hybrid screening and the cloning of mGCL. (A) A diagrammatic representation of the LAP2ß bait used in the screen. (B) Summary of the results from the interaction studies performed in yeast of the indicated baits and preys. DBD, DNA binding domain; SR, specific region; TM, trans-membrane; TA, transactivation. (C) The mouse GCL (mGCL) predicted amino acid sequence and homology to Drosophila GCL. The BTB/POZ domain is boxed (amino acids 89-198). The LAP2ß interaction region is shaded grey (amino acids 138-524). The putative NLS sequences are in bold type (amino acids 48-53 and 82-87). The conserved putative tyrosine phosphorylation site is indicated by a bold asterisk (amino acid 405); the asterisk at amino acid 525 indicates the stop codon. The mGCL sequence has been submitted to the GenBank database under the accession number AF282322.
