Fig. 2. The full-length mGCL and LAP2ß bind in vitro. The mGCL and LAP2ß proteins were expressed as GST fusions in bacteria. Both proteins, together with LAP2, were translated in vitro in the presence of [³⁵S]-labelled methionine (10% of input, lanes 1, 4 and 7). Mixtures of [³⁵S]-labelled LAP2ß and GST or GST-mGCL (lanes 2 and 3, respectively), [³⁵S]-labelled LAP2 and GST or GST-mGCL (lanes 5 and 6, respectively) and [³⁵S]-labelled mGCL and GST or GST-LAP2ß (lanes 8 and 9, respectively) were incubated with glutathione-Sepharose beads (Pharmacia Biotech). The bound proteins were eluted, separated on SDS-PAGE and identified by autoradiography and western blot analysis using anti-GST (lower panel, lanes 2, 5, 8), anti-mGCL (lower panel, lanes 3 and 6) or anti-LAP2ß (lane 9) antibodies.

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