Fig. 4. Endogenous mGCL is expressed in pancreas and rat insulinoma cells and co-localizes with LAP2ß to the NE. (A) Whole-cell lysate of H1299 cells, transfected and untransfected with recombinant full-length mGCL (lanes1 and 2, respectively), whole-cell lysates of RIN cells (lanes 3 and 5), and mouse pancreatic tissue extracts (lanes 4 and 6) were separated on SDS-PAGE and analysed by western blot using either anti-mGCL (lanes 1-4) or anti-LAP2ß (lanes 5, 6) antibodies. (B) RIN cells were stained with anti-mGCL antibodies and analysed by confocal microscopy. Progressive serial sections of a nucleus are shown. (C) RIN cells were stained with both anti-mGCL and anti-LAP2ß antibodies and analysed by confocal microscopy.
