Fig. 5. LAP2ß and GCL have distinct biochemical fractionation properties. Cytosolic and nuclear fractions of RIN cells expressing endogenous GCL were prepared by lysis of transfected cells in hypotonic buffer. Nuclei were further extracted using either 8 M urea or a combination of Triton X-100 plus 250 mM or 500 mM NaCl, as indicated. The various extracts were separated by SDS-PAGE and analysed by western blot using anti-mGCL or anti-LAP2ß antibodies, as indicated. Cyt, cytosol; NP, nuclear pellet; NS, nuclear supernatant.
