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Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells

Exing Wang1, Janice G. Pennington1, James R. Goldenring2, Walter Hunziker3 and Kenneth W. Dunn1

11Department of Medicine, Division of Nephrology, Indiana University Medical Center, Indianapolis, IN 46202, USA 2Departments of Medicine, Surgery and Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta VA Medical Center, Augusta, GA 30912, USA 3Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609 Department of Medicine, Division of Nephrology, Indiana University Medical Center, Indianapolis, IN 46202, USA
2Departments of Medicine, Surgery and Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta VA Medical Center, Augusta, GA 30912, USA
3Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609




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Movie 1A

Polarized MDCK cells incubating in apical Alexa488-Tf and basolateral Alexa568-Tf.





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Movie 1B

Polarized MDCK cells incubating in apical Alexa488-Tf and basolateral Alexa568-Tf after treatment with BFA. .





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Movie 2A

Polarized MDCK cells incubating in basolateral Oregon Green-IgA and TxR-Tf.





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Movie 2B

Polarized MDCK cells incubating in basolateral Oregon Green-IgA and TxR-Tf after treatment with BFA





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Movie 3A

Polarized MDCK cells stably expressing GFP-Rab25.





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Movie 3B

Polarized MDCK cells stably expressing GFP-Rab25 treated with BFA.





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Movie 4A

Polarized MDCK cells incubating in basolateral FR-Tf.





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Movie 4B

Polarized MDCK cells incubating in basolateral FR-Tf following treatment with BFA.





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Movie 5

Polarized MDCK cells incubating in basolateral Alexa488-Tf and diI-LDL following treatment with BFA.





This Article
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