Fig. 5. BFA induces tubulation of endosomes associated with GFP-Rab25. 3-D stereopair images of living, polarized MDCK cells stably expressing GFP-Rab25 were collected for untreated cells (A) and cells following 40 minutes incubation in BFA (B). In untreated cells GFP-Rab25 labels punctate compartments, particularly at cell apices, whereas in cells treated with BFA, GFP-Rab25 is found on tubular compartments that extend vertically throughout the cells. Panel A is 24 µm in height, Panel B is 36 µm in height.
