Fig. 3. In transfected epithelial cells, syn1A, but not syn3, is retained intracellularly in the absence of munc18-1. Non-polarized MDCK (A,A',B) and Caco-2 cells (C,D,E,E') were fixed 24 hours after transfection with syn1A (A,A',C,D,E,E'), or syn3 (B). The cells were immunolabeled using an anti-syn1 monoclonal antibody (B,C) or polyclonal antibodies against syn3 (D). In A,A' and E,E' the cells were double immunolabeled using the monoclonal antibodies against syn1 and a polyclonal antibodies against giantin (A' GIA) and galactosyltransferase (GAL, E'). The cells were analyzed by confocal immunofluorescence microscopy. In A,A' and E,E', asterisks indicate syn 1A transfected cells showing a reticular-like distribution of the t-SNARE (in A) and Golgi complex dispersion (A'). Bars, 20 µm (A,A',D,E,E'); 10 µm (B,C).
