Fig. 4. Syn1A transport to the cell surface is rescued by coexpression of munc-18 proteins. Non-polarized MDCK cells were fixed 5 (A-A'') or 48 hours (B-C') after the coexpression of syn1A and munc-18-1 (A-B') or munc-18-2 (C,C'). Cells were double-immunolabeled using the anti-syn1 (SYN1) antibody together with the anti-munc-18-1 (M-18-1), or anti-giantin (GIA) polyclonal antibodies. In C,C', asterisks label cells with syn1A at the plasma membrane and no disturbance of the Golgi complex. Bars, 10 µm.
