Fig. 2. Effect of affinity of invasin binding on bead phagocytosis and CL-1 induction. RSF were incubated for 2 or 24 hours with beads coated with MBP (nonspecific binding control), INV497D911E (low-affinity binding) or INV497 (high-affinity binding). Bead phagocytosis was analyzed after 3 hours and CL-1 expression was measured in the supernatant by slot blot after 24 hours. (A) Quantitative representation of bead binding and uptake after 3 hours and CL-1 expression after 24 hours for each type of coating. (B) Micrographs of the phagocytosis assay. The green beads are outside and the red beads are inside the cells. Error bars represent ± s.e.m. of 3 independent experiments. Bar, 50 µm.
