Fig. 2. Western blot and immunoprecipitation analysis of PfSec31p. (A) Lysates of E. coli expressing PfSec31(WD) (lanes a,b) and recombinant PfSec31(WD) isolated by affinity chromatography on GSH-Sepharose (lanes c,d) were subjected to SDS-PAGE (10% acrylamide) and visualised by Coomassie blue staining (lanes a,c) or transferred to nitrocellulose filters and incubated with rabbit antiserum against PfSec31(WD) followed by horse radish peroxidase (HRP)-conjugated anti-rabbit IgG and visualised using ECL (lanes b,d). (B) Saponin-lysed uninfected red blood cells (lanes a,b) or harvested saponin-lysed parasitised erythrocytes (lanes c,d) were subjected to SDS-PAGE (10% acrylamide) and stained with Coomassie blue (lane a,c) or transferred to nitrocellulose and probed with rabbit antiserum against PfSec31(WD) (lanes b,d). (C) [35S]-methionine/cysteine-labelled infected erythrocytes were solubilised in detergent and the sample was subjected to an immunoprecipitation protocol using either pre-bleed rabbit serum (lane a) or rabbit anti-PfSec31(WD) antiserum (lane b). (D) Harvested saponin-lysed parasitised erythrocytes (lanes a,b) were subjected to SDS-PAGE (10% acrylamide) and transferred to nitrocellulose and probed with either pre-bleed rabbit serum (lane a) or rabbit antiserum against PfSec31(int) (lane b). An 
160 kDa band that might correspond to a full-length monomeric form of PfSec31p in the parasite samples is marked with an arrow. Higher and lower molecular mass species are indicated with asterisks.
