Fig. 5. Comparison of the location of PfSec31p with the ER-located protein PfERC in P.-falciparum-infected erythrocytes and semiquantitative analysis of the amount of PfSec31p in the erythrocyte cytosol. (a-h) Asynchronous infected erythrocytes (K1 strain) were probed with affinity-purified rabbit anti-PfSec31(WD) antiserum followed by a fluorescein-conjugated anti-rabbit IgG (green fluorescence) and a murine antiserum recognising the ER-located protein PfERC followed by Alexa-Fluor-568-conjugated anti-mouse IgG (red fluorescence). A region of partial overlap of PfSec31p and PfERC in the parasite cytosol in (d) is marked with an arrowhead. (i-p) Asynchronous infected erythrocytes (K1 strain) were probed with rabbit anti-PfSec31(WD) antiserum (green fluorescence; i-l) or a murine anti-PfERC (red fluorescence; m-p). Four optical slices (0.6 µm) were collected by confocal microscopy from the bottom to the top of the parasitised erythrocytes.
