Fig. 8. uPA antisense oligodeoxynucleotides inhibit MDA-MB-231 cell growth and promote apoptosis. (A) MDA-MB-231 cells were treated with uPA antisense, sense or nonsense oligodeoxynucleotides for 24 hours, cultured in serum-containing medium for 24 hours and then in serum-free medium for 24 hours. uPA accumulation in serum-free conditioned medium was assessed by immunoblot analysis. (B) uPA levels in CM were quantitated by densitometry and standardized to the level observed in control cultures. (C) MDA-MB-231 cells were treated with oligodeoxynucleotides according to the protocol used to measure uPA levels. Cell growth was determined by MTT assay. The asterisk indicates a significant difference compared with the control (unpaired t-test, P<0.05, n=3). (D) MDA-MB-231 cells were treated with oligodeoxynucleotides. Cytoplasmic nucleosomes were detected by ELISA. The asterisk indicates a significant difference compared with the control (unpaired t-test, P<0.05, n=3). Values in B-D represent mean±s.e.m.
