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Fig. 2. Localization of 14-3-3 ${epsilon}$ is dependent on Cdk1 activity. Embryos were fixed and stained for DNA and 14-3-3 ${epsilon}$ . (A) Mitotic domains 1, 2 and 3 in the head region of a wild-type embryo is seen initiating M14. Nuclear localization of 14-3-3 ${epsilon}$ is apparent in these cells. (B) In a stg homozygous mutant at a similar embryonic stage, nuclear staining is absent. (C) Expression of Cdk1AF in a stg mutant promotes mitosis (DNA on the left) and concentration of 14-3-3 ${epsilon}$ near chromosomes in metaphase (on the right). (D) In a metaphase arrest induced by non-degradable cyclin A (A^s) and non-degradable cyclin B (B^s), 14-3-3 ${epsilon}$ (green) remains concentrated near chromosomes (purple). (E) In an irradiated embryo in which cells are delayed from entering M14, 14-3-3 ${epsilon}$ remains cytoplasmic. Bar, 16.5 µm.

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