Fig. 1. Confocal imaging of phalloidin staining of wounded RGM1 cells. At 15 seconds (a,b), 1 minute (c,d) or 30 minutes (e,f) after scratching a monolayer in medium containing FDx, cultures were fixed and stained with TRITC-phalloidin. Shown are paired, confocal microscope images of fluorescein fluorescence indicating cell permeation with FDx as a result of a plasma membrane disruption event (a,c,e) and rhodamine fluorescence indicating F-actin distribution (b,d,f). At 15 seconds post-disruption-injury, FDx-labeled zones of cytoplasm bordering on the scratch site were (a) often apparently depleted of F-actin (b, arrowhead). By contrast, at 1 or 30 minutes post disruption-injury, FDx labeled cells that had survived a disruption (c,e) characteristically displayed very strong phalloidin staining in cortex bordering on the scratch site (d,f, arrowheads). Bar, 10 µm. W, wounded; NW, not wounded.
