Fig. 8. Contraction of FPCL is induced following exposure of HFL-1 to function-blocking antibodies against VN integrins. (A) Fibroblast populated collagen gels were exposed to culture medium alone (
), P1F6 (
), MAPK inhibitor PD98059 (
) or P1F6 in the presence of PD98059 (
), PKC inhibitor calphostin c, PI-3 kinase inhibitor wortmannin, Rho-GTPases inhibitor C3-exoenzyme, F-actin inhibitor cytochalasin D or tyrosine kinase inhibitor genestein (all 
). Gel dimensions were determined after 24, 48 and 72 hours. (B) Fibroblast populated collagen gels were exposed to culture medium alone () or VN 1 µg/ml () or 10 µg/ml (). Gel dimensions were determined after 24, 48 and 72 hours. Results are calculated from duplicate wells of at least four different experiments and are expressed as mean±s.e.m. *Significantly less gel area compared to cells exposed to medium alone, P<0.05.
