Fig. 5. The D2 receptor isoforms are not found in the transferrin endocytosis-pathway. The intracellular labeling obtained with rhodamine-labeled transferrin either after 10 minutes (C) or 60 minutes (F) incubation at 37°C does not match that of the tagged-D2b receptor revealed by the monoclonal 9E10 antibody coupled to FITC (A,D) at the same incubation time. Superimposition of confocal images of the transferrin and D2 receptor labeling (B,E) clearly shows that the two types of labeling are mutually exclusive. Confocal microscope images; bar, 2 µm.
