Fig. 7. Noradrenaline and PE but not isoproterenol (ISO) potentiate the induction of the hsp70 gene promoter and increase thermotolerance in oyster immune cells subjected to heat-shock. (A) The heat (41°C)-induced increase in luciferase activity is significantly higher in oyster hemocytes pretreated with NA or PE but not in cells pretreated with isoproterenol. (B) Cell survival after severe heat-treatment (45°C, 60 minutes) is significantly higher in oyster hemocytes pretreated with NA or PE than in control samples incubated in the absence of drugs (Bas). Cell survival is not significantly different in cells pretreated with isoproterenol. Data are means and standard errors of three replicate experiments. (a) and (b) above the error bars denote significant (one letter for P<0.05, two letters for P<0.01) differences from non-heat-shocked samples (a) or heat-shocked samples (b) incubated in the absence of drugs (Bas).
