Fig. 3. Thin-section electron micrographs of yeast cells with aggregated mitochondria. Wild-type (A) and the temperature-sensitive ssc1 mutant cells (ssc1-2, B,C; ssc1-3, D) were grown at 23°C in YPD medium. Aliquots from the cells grown at 23°C were shifted to 37°C for 60 minutes. Cells were prepared for electron microscopy by the permanganate fixation method as described in Materials and Methods. Arrows in panel A show mitochondria. M in panels B-D shows regions where mitochondria cluster. N, nuclei. Bars, 1 µm.
