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Fig. 8. Quantification of each intermediate at each stage of mitosis. Data are presented as a percentage of the total NPC-like structures at each stage. The stage of mitosis is defined on the right-hand side with an example of a nucleus used in the quantification; the intermediate structures quantified are shown along the bottom. No data is given for metaphase (c) as these could not be visualized by FESEM. This shows that mature NPCs predominate in interphase (a) but disappear rapidly in prophase (b), in which star rings predominate. In early telophase (d), early assembly intermediates (particularly dimples and pores) predominate, whereas, in late telophase (e), assembly appears to progress to star rings and then thin rings in early interphase (f).





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