Fig. 7. MDC-vacuoles are labeled with LC3 a protein that associates with autophagosome membranes. CHO cells were transfected with pEGFP-LC3 as described in Materials and Methods. After 48 hours transfection cells were incubated for two hours at 37°C in 
-MEM medium (control), EBSS medium (starvation), or EBSS medium containing 50 µM vinblastine. Following this incubation period, cells were incubated with MDC at 0.05 mM for 10 minutes at 37°C and then washed four times with PBS pH 7.4. Cells were immediately analyzed by fluorescence microscopy. Typical images are shown. Insets: MDC-labeled vacuoles (red) are decorated with GFP-LC3 (green).
