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Fig. 3. Triple labeling with annexin V-fluo (green), for titin (red) and DNA (DAPI, blue) of C2C12 (A1,A2) and H9C2 (B1,B2) myoblasts shows that annexin V labels mainly early differentiating muscle cells with a punctate titin expression pattern (A1,B1, arrows). At later stages of differentiation, when the titin-expression pattern becomes filamentous, myoblasts were almost completely negative for annexin V-fluo (A2,B2). Bar, 20 µm.
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