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Fig. 1. Characterization of new antibodies against POM121. (A) Immunostaining of monolayer BRL cells with anti-POM121 antibodies. A grazing section (a), an equatorial section (b) and the corresponding DNA staining (c) of nuclei of BRL cells are shown. Bar, 20 µm. (B) Western blotting of SDS-PAGE separated proteins of rat nuclear envelope membranes extracted with 7 M urea (7M up) or of whole cell lysates of monolayer cultures of BRL cells (BRL lyste), using anti-POM121 antibodies.





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