Fig. 3. Proteolytic breakdown of POM121 during apoptosis induced by UV-irradiation. Monolayer cultures of BRL cells were exposed to UV-irradiation and incubated for 24 hours. (A) Fluorescence micrographs of UV-irradiated BRL cells showing three cells in different stages of apoptotic progression. Control cells were similar to those in Fig. 2A panels c,d. Note the almost complete lack of POM121 immunostaining in the cell with condensed chromatin in the nuclear periphery (upper right). Bar, 20 µm. (B) Western blot of SDS-PAGE separated proteins of whole cell lysates of BRL cells, exposed to different doses of UV-irradiation (as indicated).
