Fig. 8. Distribution of gp210 in apoptotic cells lacking POM121. BRL cells were treated with 1 µM staurosporine for 15 hours and subjected to double immunolabeling using antibodies specific for POM121 (a,d) and gp210 (b,e), respectively. DNA was visualized using Hoechst 33258 (c,f). Control cells (a-c) displayed a punctate fluorescence pattern along the nuclear rim. The gp210 antibodies also gave rise to a background staining seen as small dots in the nucleoplasm. In staurosporine-treated cells (d-f) both antibodies gave rise to a bright peripheral immunostaining of a type I cell (left), reminiscent of control cells. By contrast, the nuclear periphery of an early type II cell (right) only stained positive for gp210 (e), whereas POM121 was absent (d). Bar, 20 µm.
