Fig. 1. Molecular anatomy of wild type (wt) mDia1 and the deletion fragments used in this study. Location and extension of the domains and motifs within the polypeptide, as compiled from several studies, are indicated and colour-coded. For references see text. RBD, Rho-binding domain, comprising amino acid residues 63-260; FH1 (aa residues 571-735), FH2 (aa 945-1010), FH3 (aa 157-456), formin homology domains 1, 2 and 3, respectively. The two coiled coil regions are 1st CCD (aa 457-570) and 2nd CCD (aa 1011-1192). Within the C-terminal intramolecular interaction domain (CIID; aa 1116-1255), the highly conserved diaphanous-related formin autoregulatory domain (DAD; aa 1177-1207) has been identified.
