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Fig. 2. Distribution of IP3Rs, Ca2+ATPase and {alpha}-actinin in single optical sections (750±75 nm). Top panels: the SR labels for both IP3R (left panel) and Ca2+ATPase (middle panel) in a cross-striated pattern; the cross striations are superimposable (yellow in right panel). By contrast, only anti-IP3R stains the nuclear envelope region. Bottom panels: a different polyclonal anti-IP3R antibody (IP3R-M) (left panel) also reveals cross-striated staining. Middle panel, {alpha}-actinin staining pattern; right panel, overlay of the two images.





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