Fig. 3. Colocalization of muscle proteins and IP₃R using confocal microscopy. (A,B,C) IP₃R localized in the same region as -actinin of the Z line (single section, 710±71 nm). The composite, C, shows the yellow resulting from superimposition of green and red. (D,E,F) IP₃R and myosin are found in alternating cross-striated bands. Panels are images of a single optical section (670±67 nm) showing IP₃R (D), myosin fast form (E) and overlay (F). (G) Mature patterns of IP₃R and ₁ DHPR localization, in a single optical section (340±34 nm), showing overlay of green IP₃Rs and red ₁ DHPRs. Note that the dots (₁) often overlap (arrows) or border (arrowheads) the edges of the IP₃R striations.

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