Fig. 2. Spatial distribution of PML bodies relative to the MHC and EDC genomic regions. Immuno-FISH was performed on three-dimensionally preserved fibroblast nuclei, detecting PML bodies by immunofluorescence (blue) and simultaneously visualising the MHC using genomic probes recognising the LMP/TAP region (green) and the EDC using genomic probes recognising the SPRR region (red). Projections of serial optical sections through each nucleus are shown. PML bodies can be observed either closely associated or overlapping with both the MHC and the EDC in a percentage of the nuclei investigated. A-D demonstrates the variability in PML body association, with PML bodies found in association with both MHC regions (A), both EDC regions (B), associating with one MHC region and one EDC region (C) and associating with neither genomic region (D).
