Fig. 6. Comparison between intracellular localization of endogenous nSMase 1 and overexpressed GFP-nSMase 1 in normal rat fibroblast 3Y1 cells. (A) Phase-contrast of 3Y1. (B) Cells in A stained for nSMase 1 with anti-rnSMase 1 antibody (green). (C) Cells in A stained for cytoplasm with anti-KDEL antibody (red). (D) Cells in A stained for nuclear DNA with DAPI. (E) Phase contrast of 3Y1. (F) Control staining of cells in E with the antibody pre-treated with purified rat nSMase 1. (G) Phase contrast of 3Y1 cells in H. (H) Fluorescence of 3Y1 cells transformed with plasmid expressing GFP-nSMase 1 (green).
