Fig. 3. Endocytosis of CT after treatment of non-induced (filled bars) and induced (open bars) BHK antisense CHC cells with different inhibitors. The cells were preincubated with cytochalasin D (10 µg/ml), genistein (100 µg/ml) and filipin (5 µg/ml) in Hepes-buffered MEM medium for 30 minutes at 37°C, before TAG-and biotin-labeled CT (9 ng/ml) was added, and the cells were incubated at 37°C for 20 minutes in the presence of the different inhibitors. Endocytosed CT was quantified as described in Materials and Methods, and is presented here as percent of untreated control. The values are mean ± s.d. (n=4). The amount of CT associated with the cells was unaffected by the inhibitors added. Absolute values of controls for non-induced cells were in the range 31-48%, and for induced cells 8-19%.
