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Fig. 3. PLC{delta} PH domain-GFP relocalization in chlorpromazine. (a) Confocal fluorescent image of membrane localization of PLC{delta} PH domain-GFP fusion protein in transfected NIH-3T3 cells. The fluorescent intensities along the white lines were plotted as line intensity histograms, shown in the right panels. Calculation of Ipm/Icyt was used to quantify the extent of membrane localization. (b) Localization of PLC{delta} PH domain-GFP (left panel) and fluorescence intensity changes (right panel) after incubation with 30 µM chlorpromazine.





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