Fig. 4. Time course of PIP2 relocalization and membrane tension in the presence of local anesthetics. (a) Fluorescence ratios calculated from line intensity plots (see legend to Fig. 3) were plotted against time. Chlorpromazine or met-chlorpromazine was added at 0 seconds, and images were taken at 5 minute intervals. (b) Tether force was measured as described in Fig. 1 and plotted against time. Chlorpromazine or met-chlorpromazine was added at 0 seconds, and measurements were made at 5 minute intervals. CPZ, chlorpromazine; M-CPZ, met-chlorpromazine.
