Fig. 7. The effect of 30 µM chlorpromazine on phospholipid metabolism, as measured by HPLC. Phosphatidylinositol-lipids in NIH-3T3 fibroblasts were radiolabeled with [3H]inositol during a 24 hour period. Incubation and lipids were extracted from control cells and cells treated with chlorpromazine. (a) Typical HPLC radio-trace of separated phosphatidylinositols from control cells. (b) HPLC trace of phosphatidylinositol-lipids separated from cells after 30 minutes exposure to 30 µM chlorpromazine. (c) Bar chart representing summary of HPLC data. Integrated intensity of the PI4 or PIP2 peak after chlorpromazine treatment was calculated for each sample, normalized to control value and plotted as relative count per minute (CPM).
