|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. Affinity purification of the apoptotic fragments of PARP-1. Bovine PARP-1 (purified to homogeneity) was cleaved with caspase 3 and subjected to ssDNA-cellulose affinity chromatography as described in Material and Methods. Silver-stained SDS-polyacrylamide gel (Desnoyers et al., 1995) showing the apoptotic fragments of PARP-1 obtained in late (
500 mM NaCl; 24 kDa) and early (89 kDa) fractions of the chromatography procedure.
|
