Fig. 2. The phospho-FIGQY polyclonal antibody labels developing nervous system and epithelia in neuroglian-expressing but not neuroglian-null Drosophila embryos. Drosophila heterozygotes (A-D) or neuroglian null mutants (E-H), were examined by triple labeling immunofluorescence with anti-HRP as an antibody penetration control (A,E), anti-neuroglian to distinguish wild type from neuroglian null flies (B,F), and the phospho-FIGQY polyclonal antibody (C,G). Neuroglian nulls exhibited no phospho-FIGQY reactivity. Composite images are shown in (D,H) with HRP signal indicated in blue. Arrowheads mark the nervous system and brackets mark the epidermal epithelium. Scale bar: 25 µm.
