Fig. 6. FIGQY-phosphorylated L1 CAMs are localized to adherens junctions in vertebrate and Drosophila epithelial tissues. Drosophila embryos at stage 12-15 were labeled with a neuroglian antibody (B), demonstrating the presence of neuroglian in the basolateral domain of the hindgut. Labeling the same embryo with the phospho-FIGQY polyclonal antibody (A) revealed FIGQY-phosphorylated neuroglian concentrated in apicolateral regions. Composite image is shown in C. Embryos were also labeled with the adherens junction marker armadillo (E) and the phospho-FIGQY polyclonal antibody (D). A composite image is shown in F, with areas of overlap in yellow. (G) A section of E15 rat lung was labeled with the phospho-FIGQY polyclonal antibody demonstrating labeling at adherens junctions. A higher magnification image of the boxed area in G is shown in H. (I) The phospho-FIGQY polyclonal antibody signal is concentrated apically in the developing midgut of the E15 rat. Adherens junctions could not be resolved in these sections. NrCAM shows a similar distribution (J). Scale bars: 10 µm in A-C,G,I,J; 5 µm in D-F.
