Fig. 1. Assignment of colocalisation coefficient to Cx-GFP expressing cells. Real time trafficking of Cx26-GFP was recorded every 30 seconds over a 30 minute time period. Time points 1 (30 seconds, t1), 30 (15 minutes, t2) and 60 (30 minutes, t3) were assigned red (A), green (B) and blue (C) channels, respectively. Merging the three images gives a view of the vesicular trafficking in the cell (D). (E) Representative fluorogram of the extent of colocalisation of red and blue channels where the line of identity is indicated (see Materials and Methods for details). Ce, colocalisation coefficient. Bar, 5 µm. Arrows indicate vesicular movement between the three time points.
