Fig. 2. The effect of inhibitors of the secretory pathway and cytoskeleton on intercellular transfer of Lucifer yellow. HeLa cells expressing the various connexin proteins were assessed for their ability to transfer Lucifer yellow across gap junctions as described in Materials and Methods. The results are expressed as the percentage of cells transferring dye to >2 neighbouring cells ±s.e.m. At least 90 cells were injected per experiment, n=3. The effect of 15°C and cytochalasin D on cells expressing wtCx32 and Cx32I28L was not determined (Nd). Statistical significance was determined by Student t-test analysis. P<0.05 was considered significant. *P<0.05; **P<0.001; *** P<0.0005.
