Fig. 6. The effect of BFA and nocodazole on vesicular trafficking of Cx43-GFP and Cx26-GFP. Images were analysed as described in Fig. 1 before and after treatment of the cells with BFA or nocodazole for 5 hours. Red, green and blue vesicles indicate vesicular trafficking, a white image indicates colocalisation of the three time points. Cx43-GFP (A) and Cx26-GFP (B) under normal conditions; Cx43-GFP (C) and Cx26-GFP (D) following BFA treatment; Cx43-GFP (E) and Cx26-GFP (F) following nocodazole treatment. Arrows point to gap junctions at the plasma membrane.
