Fig. 1. (A) Schematic overview of murine ARVCF with the N-terminal coiled-coil domain and the central armadillo repeat region (GenBank accession no. AJ243418). The positions of the three gene-specific primer sets generating novel isoforms when used in RT-PCR are marked with arrows. (UTR, untranslated region). (B) Schematic drawing of the two N-terminal and four C-terminal splice variants of murine ARVCF obtained by RT-PCR. Exons (E) were postulated by comparing the known mouse and human ARVCF cDNA sequences with the human genomic ARVCF clone (AC005663). Numbers of the exons correspond to the human sequence. (C) Amino acid sequence comparison of the N- and C-terminal isoforms of mARVCF. The asterisks mark the stop codons; the putative PDZ-binding domain in isoform 3/7 is underlined.
